2020 Publications MIC Faculty and Students

A cytoskeleton regulator AVIL drives tumorigenesis in glioblastoma.
Xie Z, Janczyk PŁ, Zhang Y, Liu A, Shi X, Singh S, Facemire L, Kubow K, Li Z, Jia Y, Schafer D, Mandell JW, Abounader R, Li H. Nat Commun. 2020 Jul 10;11(1):3457. doi: 10.1038/s41467-020-17279-1. PMID: 32651364; PMCID: PMC7351761.

Gene expression in mouse muscle over time after nickel pellet implantation.
Bannon DI , Bao W , Turner SD , McCain WC , Dennis W , Wolfinger R , Perkins E , Abounader R . Metallomics. 2020 Apr 1;12(4):528-538. doi: 10.1039/c9mt00289h. Epub 2020 Feb 17. PMID: 32065191.

Deletion of the SAPS1 subunit of protein phosphatase 6 in mice increases radiosensitivity and impairs the cellular DNA damage response.
Dziegielewski J, Bońkowska MA, Poniecka EA, Heo J, Du K, Crittenden RB, Bender TP, Brautigan DL, Larner JM. DNA Repair (Amst). 2020 Jan;85:102737. PMID: 31751917.

Aurora B Kinase Promotes CHIP-Dependent Degradation of HIF1α in Prostate Cancer Cells.
Biswas K, Sarkar S, Said N, Brautigan DL, Larner JM. Mol Cancer Ther. 2020 Apr;19(4):1008-1017. doi: 10.1158/1535 7163.MCT-19-0777. PMID: 31848297.

Protein kinase CK2 phosphorylation of SAPS3 subunit increases PP6 phosphatase activity with Aurora A kinase.
Heo J, Larner JM, Brautigan DL. Biochem J. 2020 Jan 31;477(2):431-444. doi: 10.1042/BCJ20190740. PMID: 31904830.

Using Imaging Flow Cytometry to Quantify Neutrophil Phagocytosis.
Smirnov A, Solga MD, Lannigan J, Criss AK. Methods Mol Biol. 2020;2087:127-140. doi: 10.1007/978-1-0716-0154-9_10. PMID: 31728988; PMCID: PMC7003993.

Imaging Flow Cytometry Analysis of CEACAM Binding to Opa-Expressing Neisseria
gonorrhoeae.
Werner LM, Palmer A, Smirnov A, Belcher Dufrisne M, Columbus L, Criss AK. Cytometry A. 2020 Jun 2. doi: 10.1002/cyto.a.24037. Epub ahead of print. PMID: 32484607.

Murine Endometrial Organoids to Model Chlamydia Infection.
Bishop RC, Boretto M, Rutkowski MR, Vankelecom H, Derré I. Front Cell Infect Microbiol. 2020 Aug 14;10:416. doi: 10.3389/fcimb.2020.00416. PMID: 32923409; PMCID: PMC7456808.

Host and Bacterial Glycolysis During Chlamydia trachomatis Infection.
Ende RJ, Derré I. Infect Immun. 2020 Sep 8:IAI.00545-20. doi: 10.1128/IAI.00545-20. Epub ahead of print. PMID: 32900818.

Models and molecular mechanisms of blood vessel co-option by cancer cells.
Zhang Y, Wang S, Dudley AC. Angiogenesis. 2020;23(1):17-25. doi:10.1007/s10456-019-09684 PMID: 31628560 PMCID: PMC7018564

Reporter mice for isolating and auditing cell type-specific extracellular vesicles in vivo.
McCann JV, Bischoff SR, Zhang Y, Cowley DO, Sanchez-Gonzalez V, Daaboul GD, Dudley AC. Genesis. 2020 Jul;58(7):e23369. doi: 10.1002/dvg.23369. Epub 2020 Jun 16. PMID: 32543746; PMCID: PMC7405599.

Macrophage hypophagia as a mechanism of innate immune exhaustion in mAb-induced cell clearance.
Pinney JJ, Rivera-Escalera F, Chu CC, Whitehead HE, VanDerMeid K, Nelson AM, Barbeau MC, Zent CS, Elliott MR. Blood. 2020 Jun 17:blood.2020005571. doi: 10.1182/blood.2020005571. Epub ahead of print. PMID: 32556153.

High-resolution quantification of discrete phagocytic events by live cell time-lapse high-content microscopy imaging.
Chu CC, Pinney JJ, Whitehead HE, Rivera-Escalera F, VanDerMeid KR, Zent CS, Elliott MR. J Cell Sci. 2020 Mar 5;133(5):jcs237883. doi: 10.1242/jcs.237883. PMID: 32005699; PMCID: PMC7075070.

Measuring Leukocyte Migration to Nucleotides.
Moon TJ, Elliott MR. Methods Mol Biol. 2020;2041:345-349. doi: 10.1007/978-1-4939-9717-6_26. PMID: 31646502.

Ebola Virus Inclusion Body Formation and RNA Synthesis Are Controlled by a Novel Domain of Nucleoprotein Interacting with VP35.
Miyake T, Farley CM, Neubauer BE, Beddow TP, Hoenen T, Engel DA. J Virol. 2020 Jul 30;94(16):e02100-19. doi: 10.1128/JVI.02100-19. PMID: 32493824; PMCID: PMC7394894.

Immunomodulation of intracranial melanoma in response to blood-tumor barrier opening with focused ultrasound.
Curley CT, Stevens AD, Mathew AS, Stasiak K, Garrison WJ, Miller GW, Sheybani
ND, Engelhard VH, Bullock TNJ, Price RJ. Theranostics. 2020 Jul 11;10(19):8821-8833. doi: 10.7150/thno.47983. PMID: 32754281; PMCID: PMC7392000.

MHC-restricted phosphopeptide antigens: preclinical validation and first-in-humans clinical trial in participants with high-risk melanoma.
Engelhard VH, Obeng RC, Cummings KL, Petroni GR, Ambakhutwala AL, Chianese-Bullock KA, Smith KT, Lulu A, Varhegyi N, Smolkin ME, Myers P, Mahoney KE, Shabanowitz J, Buettner N, Hall EH, Haden K, Cobbold M, Hunt DF, Weiss G, Gaughan E, Slingluff CL Jr. J Immunother Cancer. 2020 May;8(1):e000262. doi: 10.1136/jitc-2019-000262. PMID: 32385144; PMCID: PMC7228659.

Variability in the B cell-receptor repertoire across immune-mediated diseases.
Rovin BH, Erickson LD. Kidney Int. 2020 Sep;98(3):536-538. doi: 10.1016/j.kint.2020.04.029. PMID: 32828231.

B Cell Responses in the Development of Mammalian Meat Allergy.
Chandrasekhar JL, Cox KM, Erickson LD. Front Immunol. 2020 Jul 17;11:1532. doi: 10.3389/fimmu.2020.01532. PMID: 32765532; PMCID: PMC7379154.

Phosphorylation of Toxoplasma gondii Secreted Proteins during Acute and Chronic Stages of Infection.
Young JC, Broncel M, Teague H, Russell MRG, McGovern OL, Renshaw M, Frith D, Snijders AP, Collinson L, Carruthers VB, Ewald SE, Treeck M. mSphere. 2020 Sep 9;5(5):e00792-20. doi: 10.1128/mSphere.00792-20. PMID: 32907954.

The molecular biology and immune control of chronic Toxoplasma gondii infection.
Zhao XY, Ewald SE. J Clin Invest. 2020 Jul 1;130(7):3370-3380. doi:10.1172/JCI136226. PMID: 32609097; PMCID: PMC7324197.

IL-1R Regulates Disease Tolerance and Cachexia in Toxoplasma gondii Infection.
Melchor SJ, Saunders CM, Sanders I, Hatter JA, Byrnes KA, Coutermarsh-Ott S, Ewald SE. J Immunol. 2020 Jun 15;204(12):3329-3338. doi: 10.4049/jimmunol.2000159. Epub 2020 Apr 29. PMID: 32350081; PMCID: PMC7323938.

Automated Spatially Targeted Optical Microproteomics (autoSTOMP) to Determine Protein Complexity of Subcellular Structures.
Yin B, Mendez R, Zhao XY, Rakhit R, Hsu KL, Ewald SE. Anal Chem. 2020 Jan 21;92(2):2005-2010. doi: 10.1021/acs.analchem.9b04396. Epub 2020 Jan 7. PMID: 31869197.

Unravelling the pathophysiology of vascular leakage induced by Daboia russelli snake venom.
Rucavado A, Escalante T, Camacho E, Gutiérrez JM, Fox JW. Toxicon. 2020 Apr 20;177 Suppl 1:S47-S48. PMID: 32635054.

Importance of metalloproteinases in envenoming by Bothrops atrox, in the Brazilian Amazon: Local damage approaches.
Gimenes SNC, Colombini M, Dos Santos Ibiapina HN, Costa AG, Sachett J, Monteiro WM, Wen FH, Fox JW, da Silva AMM. Toxicon. 2020 Apr 20;177 Suppl 1:S56 PMID: 32635091.

Local damage in human envenomings by Bothrops atrox in Brazilian Amazon.
Gimenes SNC, Colombini M, Dos Santos Ibiapina HN, Costa AG, Sachett J, Monteiro WM, Wen FH, Fox JW, da Silva AMM. Toxicon. 2020 Apr 20;177 Suppl 1:S8. PMID: 32635127.

Analysis of wound exudates reveals differences in the patterns of tissue damage
and inflammation induced by the venoms of Daboia russelii and Bothrops asper in mice.
Rucavado A, Escalante T, Kalogeropoulos K, Camacho E, Gutiérrez JM, Fox JW.Toxicon. 2020 Aug 8;186:94-104. PMID: 32781076.

Loss of ADAM9 Leads to Modifications of the Extracellular Matrix Modulating Tumor Growth.
Abety AN, Pach E, Giebeler N, Fromme JE, Aramadhaka LR, Mauch C, Fox JW, Zigrino P. Biomolecules. 2020 Sep 7;10(9):E1290. PMID: 32906814.

PRAS40 Phosphorylation Correlates with Insulin-Like Growth Factor-1 Receptor-Induced Resistance to Epidermal Growth Factor Receptor Inhibition in Head and Neck Cancer Cells.
Dougherty MI, Lehman CE, Spencer A, Mendez RE, David AP, Taniguchi LE, Wulfkuhle J, Petricoin EF, Gioeli D, Jameson MJ. Mol Cancer Res. 2020 Sep;18(9):1392-1401. doi: 10.1158/1541-7786.MCR-19-0592. PMID: 32467173; PMCID: PMC7483558.

AR phosphorylation and CHK2 kinase activity regulates IR-stabilized AR-CHK2 interaction and prostate cancer survival.
Ta HQ, Dworak N, Ivey ML, Roller DG, Gioeli D. Elife. 2020 Jun 24;9:e51378. PMID: 32579110; PMCID: PMC7338052.

Recombinant Peptide Production Platform Coupled with Site-Specific Albumin Conjugation
Enables a Convenient Production of Long-Acting Therapeutic Peptide.
Bak M, Park J, Min K, Cho J, Seong J, Hahn YS, Tae G, Kwon I. Pharmaceutics. 2020 Apr 16;12(4):364. PMID: 32316169; PMCID: PMC7238188.

Sequence and Functional Variation in the HIV-1 Rev Regulatory Axis.
Jackson PEH, Dzhivhuho G, Rekosh D, Hammarskjold ML. Curr HIV Res. 2020;18(2):85-98. doi: 10.2174/1570162X18666200106112842. PMID: 31906839; PMCID: PMC7113094.

Development of a pseudovirus assay and evaluation to screen natural products
for inhibition of HIV-1 subtype C reverse transcriptase.
Mavhandu LG, Cheng H, Bor YC, Tebit DM, Hammarskjold ML, Rekosh D, Bessong PO. J Ethnopharmacol. 2020 Aug 10;258:112931. doi: 10.1016/j.jep.2020.112931. Epub 2020 Apr 30. PMID: 32360797.

Human endogenous retrovirus-K mRNA expression and genomic alignment data in hepatoblastoma.
Grabski DF, Ratan A, Gray LR, Bekiranov S, Rekosh D, Hammarskjold ML, Rasmussen SK. Data Brief. 2020 Jun 18;31:105895. doi: 10.1016/j.dib.2020.105895. PMID: 32637500; PMCID: PMC7330144.

Upregulation of human endogenous retrovirus-K (HML-2) mRNAs in hepatoblastoma: Identification of potential new immunotherapeutic targets and biomarkers.
Grabski DF, Ratan A, Gray LR, Bekiranov S, Rekosh D, Hammarskjold ML, Rasmussen SK. J Pediatr Surg. 2020 May 27:S0022-3468(20)30353-5. doi: 10.1016/j.jpedsurg.2020.05.022. Epub ahead of print. PMID: 32682541

The Ethanolamine-Sensing Transcription Factor EutR Promotes Virulence and Transmission during Citrobacter rodentium Intestinal Infection. Infect Immun.
Rowley CA, Sauder AB, Kendall MM. 2020 Aug 19;88(9):e00137-20. doi:10.1128/IAI.00137-20. PMID: 32631916; PMCID: PMC7440760.

RIPK3-dependent recruitment of low-inflammatory myeloid cells does not protect from systemic Salmonella infection.
Satkovich J, Anderson CJ, Medina Chris, Ottolini Matteo, Lukens J, Kendall MM*. mBio. 2020.

A trans-eQTL network regulates osteoclast multinucleation and bone mass.
Pereira M, Ko JH, Logan J, Protheroe H, Kim KB, Tan ALM, Park KS, Rotival M, Petretto E, Bassett JHD, Williams GR, Behmoaras J, 2020; eLife. 9() . PMID: 32553114

New Approaches to SCLC Therapy: From the Laboratory to the Clinic.
Poirier JT, George J, Owonikoko TK, Berns A, Brambilla E, Byers LA, Carbone D, Chen HJ, Christensen CL, Dive C, Farago AF, Govindan R, Hann C, Hellmann MD, Horn L, Johnson JE, Ju YS, Kang S, Krasnow M, Lee J, Lee SH, Lehman J, Lok B, Lovly C, MacPherson D, McFadden D, Minna J, Oser M, Park K, Park KS, Pommier Y, Quaranta V, Ready N, Sage J, Scagliotti G, Sos ML, Sutherland KD, Travis WD, Vakoc CR, Wait SJ, Wistuba I, Wong KK, Zhang H, Daigneault J, Wiens J, Rudin CM, Oliver TG, , 2020; Journal of thoracic oncology : official publication of the International Association for the Study of Lung Cancer. 15(4) 520-540. PMID: 32018053 | PMCID: PMC7263769

Reprogramming fatty acyl specificity of lipid kinases via C1 domain engineering.
Ware TB, Franks CE, Granade ME, Zhang M, Kim KB, Park KS, Gahlmann A, Harris TE, Hsu KL,2020; Nature chemical biology. () . PMID: 31932721 | PMCID: PMC7117826

Interpreting an apoptotic corpse as anti-inflammatory involves a chloride sensing pathway.
Perry JSA, Morioka S, Medina CB, Iker Etchegaray J, Barron B, Raymond MH,Lucas CD, Onengut-Gumuscu S, Delpire E, Ravichandran KS. Nat Cell Biol. 2019 Dec;21(12):1532-1543. doi: 10.1038/s41556-019-0431-1. PMID: 31792382; PMCID: PMC7140761.

Astrocytic trans-Differentiation Completes a Multicellular Paracrine Feedback Loop Required for Medulloblastoma Tumor Growth.
Yao M, Ventura PB, Jiang Y, Rodriguez FJ, Wang L, Perry JSA, Yang Y, Wahl K, Crittenden RB, Bennett ML, Qi L, Gong CC, Li XN, Barres BA, Bender TP, Ravichandran KS, Janes KA, Eberhart CG, Zong H. Cell. 2020 Feb 6;180(3):502-520.e19. doi:10.1016/j.cell.2019.12.024. Epub 2020 Jan 23. PMID: 31983537; PMCID: PMC7259679.

Pannexin 1 channels in renin-expressing cells influence renin secretion and blood pressure homeostasis.
DeLalio LJ, Masati E, Mendu S, Ruddiman CA, Yang Y, Johnstone SR, Milstein JA, Keller TCS 4th, Weaver RB, Guagliardo NA, Best AK, Ravichandran KS, Bayliss DA, Sequeira-Lopez MLS, Sonkusare SN, Shu XH, Desai B, Barrett PQ, Le TH, Gomez RA, Isakson BE. Kidney Int. 2020 Sep;98(3):630-644. doi: 10.1016/j.kint.2020.04.041. Epub 2020 May 21. PMID: 32446934; PMCID: PMC7483468.

Mosaic Analysis with Double Markers reveals IGF1R function in granule cell progenitors during cerebellar development.
Terry TT, Cheng T, Mahjoub M, Zong H. Dev Biol. 2020 Sep 15;465(2):130-143. doi: 10.1016/j.ydbio.2020.07.008. Epub 2020 Jul 19. PubMed PMID: 32697974; NIHMSID:NIHMS1616035.

Gliomas Interact with Non-glioma Brain Cells via Extracellular Vesicles.
Gao X, Zhang Z, Mashimo T, Shen B, Nyagilo J, Wang H, Wang Y, Liu Z, Mulgaonkar A, Hu XL, Piccirillo SGM, Eskiocak U, Davé DP, Qin S, Yang Y, Sun X, Fu YX, Zong H, Sun W, Bachoo RM, Ge WP. Cell Rep. 2020 Feb 25;30(8):2489-2500.e5. doi: 10.1016/j.celrep.2020.01.089. PubMed PMID: 32101730.

Astrocytic trans-Differentiation Completes a Multicellular Paracrine Feedback Loop Required for Medulloblastoma Tumor Growth.
Yao M, Ventura PB, Jiang Y, Rodriguez FJ, Wang L, Perry JSA, Yang Y, Wahl K, Crittenden RB, Bennett ML, Qi L, Gong CC, Li XN, Barres BA, Bender TP, Ravichandran KS, Janes KA, Eberhart CG, Zong H. Cell. 2020 Feb 6;180(3):502-520.e19. PubMed PMID: 31983537; PubMed Central PMCID: PMC7259679.

Whole Genome Sequencing of Spontaneously Occurring Rat Natural Killer Large Granular Lymphocyte Leukemia Identifies JAK1 Somatic Activating Mutation.
Wang TT, Yang J, Dighe S, Schmachtenberg MW, Leigh NT, Farber E, Onengut-Gumuscu S, Feith DJ, Ratan A, Loughran TP Jr, Olson TL. Cancers (Basel).2020 Jan 3;12(1):126. doi: 10.3390/cancers12010126. PMID: 31947841; PMCID: PMC7017127.

Evaluation of K18-hACE2 Mice as a Model of SARS-CoV-2 Infection.
Moreau GB, Burgess SL, Sturek JM, Donlan AN, Petri WA, Mann BJ. Am J Trop Med Hyg. 2020 Sep;103(3):1215-1219. doi: 10.4269/ajtmh.20-0762. PMID: 32723427; PMCID: PMC7470527.

Dual Function of Aar, a Member of the New AraC Negative Regulator Family, in Escherichia coli Gene Expression.
Mickey AS, Nataro JP. Infect Immun. 2020 May 20;88(6):e00100-20. doi: 10.1128/IAI.00100-20. PMID: 32253248; PMCID: PMC7240098.

Type 2 Cytokines IL-4 and IL-5 Reduce Severe Outcomes from Clostridiodes difficile Infection.
Donlan A, Simpson ME, Petri WA Jr., M.D., Ph.D. Anaerobe 2020, in press.

IL-13 Predicts the Need for Mechanical Ventilation in COVID-19 Patients.
Donlan AD, Young M, Petri WA Jr., Abhyankar M. medRxiv 2020.06.18.20134353; doi:https://doi.org/10.1101/2020.06.18.20134353

Ten simple rules for reading a scientific paper.
Carey MA, Steiner KL, Petri WA Jr. PLoS Comput Biol. 2020 Jul 30;16(7):e1008032. doi: 10.1371/journal.pcbi.1008032. PMID: 32730251; PMCID: PMC7392212.

Immune Profiling To Predict Outcome of Clostridioides difficile Infection.
Abhyankar MM, Ma JZ, Scully KW, Nafziger AJ, Frisbee AL, Saleh MM, Madden GR, Hays AR, Poulter M, Petri WA Jr. mBio. 2020 May 26;11(3):e00905-20. doi: 10.1128/mBio.00905-20. PMID: 32457246; PMCID: PMC7251209.

Gut microbiome communication with bone marrow regulates susceptibility to amebiasis.
Burgess SL, Leslie JL, Uddin J, Oakland DN, Gilchrist C, Moreau GB, Watanabe K, Saleh M, Simpson M, Thompson BA, Auble DT, Turner SD, Giallourou N, Swann J, Pu Z, Ma JZ, Haque R, Petri WA Jr. J Clin Invest. 2020 Aug 3;130(8):4019-4024. doi: 10.1172/JCI133605. PMID: 32369444; PMCID: PMC7410058.

Considering the Immune System during Fecal Microbiota Transplantation for Clostridioides difficile Infection.
Frisbee AL, Petri WA Jr. Trends Mol Med. 2020 May;26(5):496-507. doi: 10.1016/j.molmed.2020.01.009. Epub 2020 Feb 17. PMID: 32359480; PMCID: PMC7198612.

Mucosal immunity and the eradication of polio.
Donlan AN, Petri WA Jr. Science. 2020 Apr 24;368(6489):362-363. doi: 10.1126/science.abb8588. PMID: 32327582.

The Inflammasome and Type-2 Immunity in Clostridium difficile Infection.
Donlan A, Petri WA Jr. Clin Colon Rectal Surg. 2020 Mar;33(2):67-72. doi: 10.1055/s-0040-1701231. Epub 2020 Feb 25. PMID: 32104158; PMCID: PMC7042014.

Inhibition of Amebic Cysteine Proteases Blocks Amebic Trogocytosis but Not Phagocytosis.
Gilmartin AA, Ralston KS, Petri WA. J Infect Dis. 2020 Apr 27;221(10):1734-1739. doi: 10.1093/infdis/jiz671. PMID: 31999350; PMCID: PMC7184912.

Gasdermin-D-dependent IL-1α release from microglia promotes protective immunity during chronic Toxoplasma gondii infection.
Batista SJ, Still KM, Johanson D, Thompson JA, OʼBrien CA, Lukens JR, Harris TH. Nat Commun. 2020 Jul 23;11(1):3687. doi: 10.1038/s41467-020-17491-z. PMID: 32703941; PMCID: PMC7378823.

Long-‘Trm’ protection of the CNS.
Kovacs MA, Harris TH. Nat Immunol. 2020 Aug;21(8):825-826. doi: 10.1038/s41590-020-0716-3. PMID: 32572243.

ICOS-deficient and ICOS YF mutant mice fail to control Toxoplasma gondii infection of the brain.
O’Brien CA, Harris TH. PLoS One. 2020 Jan 24;15(1):e0228251. doi: 10.1371/journal.pone.0228251. PMID: 31978191; PMCID: PMC6980566.

Unsupervised machine learning reveals key immune cell subsets in COVID-19, rhinovirus infection, and cancer therapy.
Barone SM, Paul AGA, Muehling LM, Lannigan JA, Kwok WW, Turner RB, Woodfolk JA, Irish JM. bioRxiv [Preprint]. 2020 Aug 1:2020.07.31.190454. doi: 10.1101/2020.07.31.190454. PMID: 32766581; PMCID: PMC7402038.

Human TH1 and TH2 cells targeting rhinovirus and allergen coordinately promote allergic asthma.
Muehling LM, Heymann PW, Wright PW, Eccles JD, Agrawal R, Carper HT, Murphy DD, Workman LJ, Word CR, Ratcliffe SJ, Capaldo BJ, Platts-Mills TAE, Turner RB, Kwok WW, Woodfolk JA. J Allergy Clin Immunol. 2020 Sep;146(3):555-570. doi: 10.1016/j.jaci.2020.03.037. PMID: 32320734; PMCID: PMC7483727.

T-bet+ Memory B Cells Link to Local Cross-Reactive IgG upon Human Rhinovirus Infection.
Eccles JD, Turner RB, Kirk NA, Muehling LM, Borish L, Steinke JW, Payne SC, Wright PW, Thacker D, Lahtinen SJ, Lehtinen MJ, Heymann PW, Woodfolk JA. Cell Rep. 2020 Jan 14;30(2):351-366.e7. PMID: 31940481; PMCID: PMC6994188.